diff --git a/gnu/packages/python-xyz.scm b/gnu/packages/python-xyz.scm
index 825d747dd8..1f772576e2 100644
--- a/gnu/packages/python-xyz.scm
+++ b/gnu/packages/python-xyz.scm
@@ -20047,9 +20047,6 @@ (define-public python-twine
 their files and supports any packaging format (including wheels).")
     (license license:asl2.0)))
 
-(define-public python2-twine
-  (package-with-python2 python-twine))
-
 (define-public python-linecache2
   (package
     (name "python-linecache2")